Experience in the use of phylogenetic analysis in the investigation of cases of HIV-infected patients

Objective. To establish a probable source of infection using phylogenetic analysis in the investigation cases HIV infection. Materials and methods. genome sequencing was carried out kit ViroSeq (Celera). The tree constructed maximum likelihood method Mega 6 program. As comparison group, 40 samples HIV-infected patients from Krasnoyarsk region were used, as well 25 nucleotide sequences various subtypes taken international GenBank database. reliability resulting evaluated bootstrap analysis, branch rotation test for thousand random constructions. Results discussion. paper presents five epidemiological investigations with nosocomial occupational mechanism transmission region. In 2013, study conducted on three persons treated same hospital an patient. A close genetic relationship shown newly identified individuals, could not be examined due to death. Second 2013. association between diagnosed woman patient previously established status is shown. Both simultaneously medical institution. determine direction virus, duration used. 2014, after receiving care four organizations, confirmed positive. all hospitals there diagnosis Three them have been examined. absence confirmed, fourth died, material obtained him. 2015, nurse injured while caring HIV-positive Phylogenetic showed high degree these 2016, car accident, two hospitalized intensive unit. After some time, both Four at time. individuals one putative source. infections shown, alleged established. Conclusion. use molecular methods significantly improves quality accuracy deciphering epidemic chains